Format

Send to

Choose Destination
Transgenic Res. 1998 Jul;7(4):319-20.

A rapid and reliable PCR-based assay for gene transmission and sex determination in newborn transgenic mice.

Author information

1
University of Texas Health Science Center at San Antonio, Department of Cellular and Structural Biology 78284-7763, USA.

Abstract

This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), the second set for the mouse Y-chromosome-specific SRY gene, and the third set for the beta subunit of the thyroid stimulating hormone (TSH beta), an internal control. This procedure allowed us to promptly analyze pups born from transgenic founders carrying the androgen receptor transgene and, at the same time, establish the sex of the animals. The method is simple, rapid and highly reproducible.

PMID:
9859220
DOI:
10.1023/a:1008838616203
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center