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EMBO J. 1998 Dec 15;17(24):7454-68.

Control of cleavage site selection during mRNA 3' end formation by a yeast hnRNP.

Author information

1
Department of Cell Biology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland. minvielle@ubaclu.unibas.ch

Abstract

Endonucleolytic cleavage of pre-mRNAs is the first step during eukaryotic mRNA 3' end formation. It has been proposed that cleavage factors CF IA, CF IB and CF II are required for pre-mRNA 3' end cleavage in yeast. CF IB is composed of a single polypeptide, Nab4p/Hrp1p, which is related to the A/B group of metazoan heterogeneous nuclear ribonucleoproteins (hnRNPs) that function as antagonistic regulators of 5' splice site selection. Here, we provide evidence that Nab4p/Hrp1p is not required for pre-mRNA 3' end endonucleolytic cleavage. We show that CF IA and CF II devoid of Nab4p/Hrp1p are sufficient to cleave a variety of RNA substrates but that cleavage occurs at multiple sites. Addition of Nab4p/Hrp1p prevents these alternative cleavages in a concentration-dependent manner, suggesting an essential and conserved role for some hnRNPs in pre-mRNA cleavage site selection.

PMID:
9857200
PMCID:
PMC1171089
DOI:
10.1093/emboj/17.24.7454
[Indexed for MEDLINE]
Free PMC Article

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