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Neuron. 1998 Nov;21(5):1177-88.

Submillisecond kinetics of glutamate release from a sensory synapse.

Author information

1
Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg, Göttingen, Federal Republic of Germany.

Abstract

Exocytosis-mediated glutamate release from ribbon-type synaptic terminals of retinal bipolar cells was studied using AMPA receptors and simultaneous membrane capacitance measurements. Release onset (delay <0.8 ms) and offset were closely tied to Ca2+ channel opening and closing. Asynchronous release was not copious and we estimate that there are approximately 5 Ca2+ channels per docked synaptic vesicle. Depending on Ca2+ current amplitude, release occurred in a single fast bout or in two successive bouts with fast and slow onset kinetics. The second, slower bout may reflect a mobilization rate of reserve vesicles toward fusion sites that is accelerated by increasing Ca2+ influx. Bipolar cell synaptic ribbons thus are remarkably versatile signal transducers, capable of transmitting rapidly changing sensory input, as well as sustained stimuli, due to their large pool of releasable vesicles.

PMID:
9856472
DOI:
10.1016/s0896-6273(00)80634-0
[Indexed for MEDLINE]
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