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J Protein Chem. 1998 Oct;17(7):657-62.

A bovine dander allergen, comparative modeling, and similarities and differences in folding with related proteins.

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1
Department of Chemistry, University of Kuopio, Finland. santa@kummeli.uku.fi

Abstract

The most important allergenic protein in cow dander and urine is Bos d 2. It is proposed to belong to the family of lipocalins, which are proteins capable of binding small hydrophobic molecules. The allergenic properties of Bos d 2 indicate an interaction between the accessible regions of the native protein and IgE. In this work, a three-dimensional model was created for Bos d 2 by comparative modeling, and features characteristic of outlier lipocalins were observed. The protruding regions of the surface were characterized and used in predicting the possible B-cell epitopes. There is a pocket inside the core and its size is appropriate for small molecules. The model shows a hydrophilic amino acid side chain of glutamic acid 115 on the inner surface of the hole and a phenylalanine as the "gatekeeper" instead of tyrosine, which is common in experimentally modeled lipocalins.

PMID:
9853680
[Indexed for MEDLINE]
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