Format

Send to

Choose Destination
Microbiology. 1998 Nov;144 ( Pt 11):3219-28.

Functional characterization of the Erwinia chrysanthemi OutS protein, an element of a type II secretion system.

Author information

1
Laboratoire de Génétique Moléculaire des Microorganismes et des Interactions Cellulaires, CNRS-UMR 5577, INSA, Villeurbanne, France.

Abstract

Secretion of pectate lyases and a cellulase occurs in Erwinia chrysanthemi through a type II secretion machinery, the Out system. Proper insertion of the secretin OutD in the outer membrane requires the presence of OutS. OutS is an outer-membrane lipoprotein that interacts directly with OutD. Using ligand-blotting experiments, it has been shown that this interaction requires at least the 62 C-terminal amino acids of OutD. When this domain was added to the C-terminal extremity of the secreted pectate lyase PelD, the construct was stabilized by OutS but not inserted into the outer membrane. Thus, this domain is sufficient to interact with OutS but it is unable to confer the ability to be inserted into the outer membrane in the presence of OutS. A screen for outS mutants unable to secrete pectate lyases gave only mutants unable to properly localize OutD in the outer membrane and no mutant in the protection function. Thus, the interaction between OutS and OutD can probably not be abolished by the mutation of a single amino acid, and the insertion of OutD in the outer membrane may require additional proteins.

PMID:
9846757
DOI:
10.1099/00221287-144-11-3219
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Ingenta plc
Loading ...
Support Center