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Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14961-6.

Production of beta-defensins by human airway epithelia.

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1
Department of Internal Medicine, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USA.

Erratum in

  • Proc Natl Acad Sci U S A 1999 Mar 2;96(5):2569.

Abstract

Human beta-defensins (HBDs) are antimicrobial peptides that may play a role in mucosal defense. Diminished activity of these peptides has been implicated in the pathogenesis of cystic fibrosis (CF) lung disease. We show that HBD-1 and HBD-2 mRNAs are expressed in excised surface and submucosal gland epithelia from non-CF and CF patients. The pro-inflammatory cytokine interleukin-1beta stimulated the expression of HBD-2 but not HBD-1 mRNA and peptide in primary cultures of airway epithelia. HBD-1 was found in bronchoalveolar lavage (BAL) fluid from normal volunteers, CF patients, and patients with inflammatory lung diseases, whereas HBD-2 was detected in BAL fluid from patients with CF or inflammatory lung diseases, but not in normal volunteers. Both HBD-1 and HBD-2 were found in BAL fluid in concentrations of several ng/ml, and both recombinant peptides showed salt-sensitive bactericidal activity. These data suggest that in the lung HBD-2 expression is induced by inflammation, whereas HBD-1 may serve as a defense in the absence of inflammation.

PMID:
9843998
PMCID:
PMC24558
[Indexed for MEDLINE]
Free PMC Article
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