Abstract
Cholinergic hybrid mouse septal neurons SN56 were differentiated by separate and combined application of 0.001 mM all-trans-retinoic acid and 1 mM dibutyryl cAMP. Each of agents caused about twofold increase of choline acetyltransferase activity. These activatory effects were additive. Dibutyryl cAMP resulted in twofold increase of ATP-citrate lyase and acetylcholinesterase activities. Retinoic acid did not affect these enzyme activities but partially abolished activatory effects of dibutyryl cAMP. Pyruvate dehydrogenase and other enzymes of acetyl-CoA metabolism were not affected by this treatment. This work demonstrates that it is possible to rise cholinergic neurons of different expression of cholinergic and acetyl-CoA metabolism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Citrate (pro-S)-Lyase / biosynthesis*
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ATP Citrate (pro-S)-Lyase / genetics
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Acetyl Coenzyme A / metabolism*
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Acetylcholine / metabolism*
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Acetylcholinesterase / biosynthesis*
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Acetylcholinesterase / genetics
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Animals
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Bucladesine / pharmacology*
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Cell Differentiation / drug effects
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Choline O-Acetyltransferase / biosynthesis*
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Choline O-Acetyltransferase / genetics
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Cytoplasm / metabolism
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Enzyme Induction / drug effects
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Hybrid Cells / drug effects*
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Mice
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Nerve Tissue Proteins / biosynthesis*
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Nerve Tissue Proteins / genetics
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Neuroblastoma / pathology
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Septum Pellucidum / pathology
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Tretinoin / pharmacology*
Substances
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Nerve Tissue Proteins
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Tretinoin
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Bucladesine
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Acetyl Coenzyme A
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Choline O-Acetyltransferase
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ATP Citrate (pro-S)-Lyase
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Acetylcholinesterase
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Acetylcholine