Activities of enzymes of acetyl-CoA and acetylcholine metabolism in SN56 hybrid cholinergic cell line differentiated by dibutyryl cyclic AMP and all-trans retinoic acid

Folia Neuropathol. 1997;35(4):247-9.

Abstract

Cholinergic hybrid mouse septal neurons SN56 were differentiated by separate and combined application of 0.001 mM all-trans-retinoic acid and 1 mM dibutyryl cAMP. Each of agents caused about twofold increase of choline acetyltransferase activity. These activatory effects were additive. Dibutyryl cAMP resulted in twofold increase of ATP-citrate lyase and acetylcholinesterase activities. Retinoic acid did not affect these enzyme activities but partially abolished activatory effects of dibutyryl cAMP. Pyruvate dehydrogenase and other enzymes of acetyl-CoA metabolism were not affected by this treatment. This work demonstrates that it is possible to rise cholinergic neurons of different expression of cholinergic and acetyl-CoA metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Citrate (pro-S)-Lyase / biosynthesis*
  • ATP Citrate (pro-S)-Lyase / genetics
  • Acetyl Coenzyme A / metabolism*
  • Acetylcholine / metabolism*
  • Acetylcholinesterase / biosynthesis*
  • Acetylcholinesterase / genetics
  • Animals
  • Bucladesine / pharmacology*
  • Cell Differentiation / drug effects
  • Choline O-Acetyltransferase / biosynthesis*
  • Choline O-Acetyltransferase / genetics
  • Cytoplasm / metabolism
  • Enzyme Induction / drug effects
  • Hybrid Cells / drug effects*
  • Mice
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Neuroblastoma / pathology
  • Septum Pellucidum / pathology
  • Tretinoin / pharmacology*

Substances

  • Nerve Tissue Proteins
  • Tretinoin
  • Bucladesine
  • Acetyl Coenzyme A
  • Choline O-Acetyltransferase
  • ATP Citrate (pro-S)-Lyase
  • Acetylcholinesterase
  • Acetylcholine