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Appl Microbiol Biotechnol. 1998 Oct;50(4):455-8.

A Pseudomonas aeruginosa biosensor responds to exposure to ultraviolet radiation.

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1
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater 74078, USA.

Abstract

We fused the Pseudomonas aeruginosa recA promoter to a promoterless Vibrio fisheri lux operon. This recA-lux fusion (pMOE15) was introduced into wild-type P. aeruginosa strain FRD1 and recA expression was monitored by measuring 490-nm light production. The RM4440 strain responded to increasing doses of ultraviolet radiation by an increase in its bioluminescence. RM4440 has the potential to be useful as a biosensor for the presence of DNA-damaging agents in the environment.

PMID:
9830096
[Indexed for MEDLINE]
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