Abstract
The synthesis of the murein precursor lipid I is performed by MraY. We have shown that mraY is an essential gene for cell growth. Cells depleted of MraY first swell and then lyse. The expression of mraY DNA in vitro produces a 40-kDa polypeptide detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / isolation & purification
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Base Sequence
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Cell Division / genetics
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Escherichia coli / cytology
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Escherichia coli / genetics*
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Escherichia coli / growth & development
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Gene Expression
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Genes, Bacterial*
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Genetic Complementation Test
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Mutagenesis, Insertional
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Phenotype
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Plasmids / genetics
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Transferases (Other Substituted Phosphate Groups)
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Transferases*
Substances
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Bacterial Proteins
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DNA Primers
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DNA, Bacterial
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Transferases
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Transferases (Other Substituted Phosphate Groups)
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mraY protein, Bacteria