Format

Send to

Choose Destination
See comment in PubMed Commons below
Biol Chem. 1998 Oct;379(10):1301-4.

Inverse expression pattern of REST and synapsin I in human neuroblastoma cells.

Author information

  • 1Medical Biochemistry and Molecular Biology, University of the Saarland, Homburg, Germany.

Abstract

The zinc finger protein REST is a repressor of neuronal genes in nonneuronal tissues. We have analyzed the expression of REST, together with the expression of a REST target gene, encoding synapsin I, in human neuroblastoma cells. It was found that REST and synapsin I are coexpressed in neuroblastoma cell lines, although the expression of REST was inversely proportional to the levels of synapsin I mRNA. Thus, increased expression of synapsin I was directly correlated with decreased expression of REST. These expression data are in excellent correlation with synapsin I promoter activity measured in neuroblastoma cells showing that an increase in the REST concentration switched off synapsin I promoter activity. We conclude that the concentration of REST determines the expression level of neuronal genes such as the synapsin I gene.

PMID:
9820593
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk