Send to

Choose Destination
J Cell Sci. 1998 Dec 18;111 ( Pt 24):3645-54.

The presenilin protein family member SPE-4 localizes to an ER/Golgi derived organelle and is required for proper cytoplasmic partitioning during Caenorhabditis elegans spermatogenesis.

Author information

Graduate Program in Biochemistry, Emory University, Atlanta, Georgia 30322, USA.


During Caenorhabditis elegans spermatogenesis, asymmetric partitioning of cellular components principally occurs via ER/Golgi-derived organelles, named fibrous body-membranous organelles. In C. elegans spe-4 mutants, morphogenesis of fibrous body-membranous organelle complexes is defective and spermatogenesis arrests at an unusual cellular stage with four haploid nuclei within a common cytoplasm. The spe-4 encoded integral membrane protein is a diverged member of the presenilin family implicated in early onset Alzheimer's disease. Specific antisera were used to show that SPE-4 resides within the fibrous body-membranous organelles membranes during wild-type spermatogenesis. Several spe-4 recessive mutants were examined for SPE-4 immunoreactivity and a deletion mutant lacks detectable SPE-4 while either of two missense mutants synthesize and localize immunoreactive SPE-4 within their fibrous body-membranous organelles. One of these missense mutations is located within a motif that is common to all presenilins. spe-4 mutants were also examined for other partitioning defects and tubulin was found to accumulate in unusual deposits close to the plasma membrane. These results suggest that wild-type SPE-4 is required for proper localization of macromolecules that are subject to asymmetric partitioning during spermatogenesis.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center