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Curr Opin Struct Biol. 1998 Oct;8(5):649-56.

Recent advances in site-directed spin labeling of proteins.

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Jules Stein Eye Institute, University of California, Los Angeles 90095, USA.


Site-directed spin labeling of proteins is experiencing a phase of rapid technical evolution, application and evaluation. New strategies have been introduced for determining membrane protein topography, electrostatic potentials, the orientation of proteins at membrane surfaces and inter-residue distances. New applications include studies of beta strands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of membrane and soluble proteins.

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