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J Infect Dis. 1998 Dec;178(6):1835-8.

Reactivity of human sera to different strains of granulocytic ehrlichiae in immunodiagnostic assays.

Author information

1
Department of Entomology, Connecticut Agricultural Experiment Station, University School of Medicine, New Haven, Connecticut, USA. louis.magnarelli@po.state.ct.us

Abstract

Sera from 35 patients diagnosed with human granulocytic ehrlichiosis in Connecticut were tested by indirect IFA staining methods with 5 strains of Ehrlichia equi or the human granulocytic ehrlichiosis agent to assess the suitability of different strains in laboratory analyses. Antigens included horse-derived infected neutrophils (MRK and BDS strains) and human isolates cultured in human promyelocytic leukemia cells (NCH-1, RCH, and Webster). Of 35 sera, 23 (65.7%) reacted to all 5 strains. Seropositivity was highest (97.1%) in assays that contained the MRK strain from California and lowest (71. 4%) in tests with the NCH-1 strain from Nantucket, Massachusetts. In parallel testing of 32 sera with the NCH-1 strain by indirect IFA and Western blot analyses, results were concordant for 30 samples (93.8%). All strains of ehrlichiae can be used in IFA analyses for antibody detection, but assay sensitivity varied with the strain used.

PMID:
9815246
DOI:
10.1086/314516
[Indexed for MEDLINE]

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