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J Bacteriol. 1998 Nov;180(22):6072-5.

The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-beta-D-thiogalactopyranoside exclusion and spectinomycin sensitivity.

Author information

1
Laboratoire des Réseaux de Régulations, Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS/URA2225, F-91405 Orsay Cedex, France.

Abstract

Expression of cloned genes from isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype. cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.

PMID:
9811673
PMCID:
PMC107689
[Indexed for MEDLINE]
Free PMC Article

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