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DNA Cell Biol. 1998 Oct;17(10):915-20.

Cloning and nucleotide sequence of amidase gene from Pseudomonas putida.

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1
DuPont Central Research and Development, Glasgow Site, Newark, DE 19714-6101, USA.

Abstract

Amidases are a class of enzymes which convert amides to acids and have potential value in the development of commercial bioprocesses for the production of useful chemicals. A gene encoding an amidase in Pseudomonas putida 5B has been cloned, sequenced, and overexpressed in Escherichia coli. An additional open reading frame (P38K) encoding a putative protein of 38 kDa was found immediately upstream of the amidase gene. This work continues our characterization of a P. putida operon, which now appears to include P38K, amidase, and a stereo-specific nitrile hydratase. This characterization underlies continuing efforts in biocatalyst development.

PMID:
9809753
DOI:
10.1089/dna.1998.17.915
[Indexed for MEDLINE]
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