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Dev Biol. 1998 Nov 15;203(2):358-68.

Suppressor of hairless activates brachyury expression in the Ciona embryo.

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1
Division of Genetics, University of California, 401 Barker Hall, Berkeley, California, 94720, USA.

Abstract

The Ciona Brachyury gene (Ci-Bra) is regulated, in part, by a 434-bp enhancer that mediates restricted expression in the notochord. Here we present evidence that a Ciona Suppressor of Hairless ¿Ci-Su(H)¿ protein functions as an activator of this enhancer. Point mutations that reduce the binding of a GST/Ci-Su(H) fusion protein in vitro diminish the expression of mutagenized Ci-Bra/lacZ transgenes in electroporated embryos. Overexpression of a Ci-Su(H) fusion protein containing the Drosophila Hairy repression domain interferes with notochord differentiation, producing mutant tadpoles with shortened tails. Expression of a constitutively activated Xotch receptor in the notochord, endoderm, and CNS also alters tail morphogenesis. These results suggest that a Notch-Su(H) pathway might participate in notochord differentiation in Ciona.

PMID:
9808786
DOI:
10.1006/dbio.1998.9067
[Indexed for MEDLINE]
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