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Eur J Immunogenet. 1998 Oct;25(5):371-4.

A novel strategy for unambiguous DPB1 typing.

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Department of Pathology, Royal Melbourne Hospital, Parkville, VIC, Australia.


A DPB1 typing method is described that assigns DPB1 alleles into six groups based on polymorphism at amino acid positions 8-9 and 84-87 using sequence-specific priming (SSP). The results obtained allow the selection of primers for a subsequent sequence-specific oligonucleotide (SSO) hybridization procedure which permits DPB1 alleles to be analysed separately in a heterozygote individual. This has greatly reduced the occurrence of typing reaction patterns consistent with multiple combinations of DPB1 alleles seen in other DPB1 typing methods.

[Indexed for MEDLINE]

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