Molecular cloning and expression of rat bradykinin B1 receptor

Biochim Biophys Acta. 1998 Nov 8;1442(2-3):177-85. doi: 10.1016/s0167-4781(98)00163-8.

Abstract

The gene encoding rat bradykinin B1 receptor has been cloned by using a partial rat B1 cDNA probe. The rat B1 receptor gene contains two exons and the entire coding region is within the second exon. The 5'-flanking region of the rat B1 receptor gene contains several putative transcriptional regulatory sites including TATA box, cAMP response element, NF-kappaB and AP-1. It showed promoter activity inducible by lipopolysaccharide in vascular smooth muscle cells. Rat B1 receptor mRNA was found to be alternatively spliced and induced by lipopolysaccharide treatment in a wide range of tissues, such as the salivary gland, testis, kidney, lung, heart, prostate and aorta. The deduced rat B1 receptor amino acid sequence is 71% homologous to human and rabbit counterparts, and 89% homologous to the mouse counterpart. The expressed B1 receptor in HEK293 cells displayed a rank order of affinity for the kinin peptides: des-Arg9-BK>Lys-des-Arg9-BK approximately des-Arg9, Leu8-BK>Sar-Tyr-epsilonAhx-Lys-[D-betaNal7, Ile8]-des-Arg9-BK>Sar-Tyr-epsilonAhx-Lys-des-Arg9-BK>>BK>> Hoe140. These results indicate that the cloned gene encodes a functional rat B1 receptor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding, Competitive
  • Cloning, Molecular / methods
  • DNA Primers
  • Humans
  • Kinetics
  • Kinins / metabolism
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rabbits
  • Rats / genetics*
  • Receptor, Bradykinin B1
  • Receptors, Bradykinin / chemistry
  • Receptors, Bradykinin / genetics*
  • Receptors, Bradykinin / metabolism
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • Kinins
  • Receptor, Bradykinin B1
  • Receptors, Bradykinin
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/U66106
  • GENBANK/U66107