Abstract
A single injection of HPV16 L1 virus-like particles induced potent CD8-mediated protection from tumor challenge by C3 cells, a line derived from embryonic mouse cells transfected with the HPV16 genome. L1 RNA, but not protein, was detected biochemically in C3 cells. These results indicate that low-level expression of HPV16 L1 can occur in proliferating cells and serve as a tumor vaccine target. Although L1 expression is generally thought to be restricted to terminally differentiated epithelial cells, these results suggest that additional analysis for low-level L1 expression in proliferating cells of HPV-induced lesions is warranted and might help in predicting the clinical potential of HPV L1 virus-like particle-based vaccines.
Copyright 1998 Academic Press.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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CD4-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / immunology
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Capsid / genetics
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Capsid / immunology
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Capsid Proteins*
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Disease Models, Animal
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Humans
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Mice
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Mice, Inbred C57BL
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Oncogene Proteins, Viral / genetics
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Oncogene Proteins, Viral / immunology*
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Papillomaviridae / genetics
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Papillomaviridae / immunology*
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Papillomavirus E7 Proteins
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Papillomavirus Infections / prevention & control*
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Tumor Cells, Cultured
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Tumor Virus Infections / prevention & control*
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Virion / immunology
Substances
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Capsid Proteins
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L1 protein, Bovine papillomavirus
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L2 protein, Bovine papillomavirus
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L2 protein, Human papillomavirus type 16
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Oncogene Proteins, Viral
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Papillomavirus E7 Proteins
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oncogene protein E7, Human papillomavirus type 16
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L1 protein, Human papillomavirus type 16