L1-specific protection from tumor challenge elicited by HPV16 virus-like particles

Virology. 1998 Oct 25;250(2):371-6. doi: 10.1006/viro.1998.9372.

Abstract

A single injection of HPV16 L1 virus-like particles induced potent CD8-mediated protection from tumor challenge by C3 cells, a line derived from embryonic mouse cells transfected with the HPV16 genome. L1 RNA, but not protein, was detected biochemically in C3 cells. These results indicate that low-level expression of HPV16 L1 can occur in proliferating cells and serve as a tumor vaccine target. Although L1 expression is generally thought to be restricted to terminally differentiated epithelial cells, these results suggest that additional analysis for low-level L1 expression in proliferating cells of HPV-induced lesions is warranted and might help in predicting the clinical potential of HPV L1 virus-like particle-based vaccines.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Capsid / genetics
  • Capsid / immunology
  • Capsid Proteins*
  • Disease Models, Animal
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Oncogene Proteins, Viral / genetics
  • Oncogene Proteins, Viral / immunology*
  • Papillomaviridae / genetics
  • Papillomaviridae / immunology*
  • Papillomavirus E7 Proteins
  • Papillomavirus Infections / prevention & control*
  • Tumor Cells, Cultured
  • Tumor Virus Infections / prevention & control*
  • Virion / immunology

Substances

  • Capsid Proteins
  • L1 protein, Bovine papillomavirus
  • L2 protein, Bovine papillomavirus
  • L2 protein, Human papillomavirus type 16
  • Oncogene Proteins, Viral
  • Papillomavirus E7 Proteins
  • oncogene protein E7, Human papillomavirus type 16
  • L1 protein, Human papillomavirus type 16