Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 1998 Oct 20;251(2):597-602.

Genomic organization and chromosomal localization of the human sepiapterin reductase gene.

Author information

1
Institute for Comprehensive Medical Science, Fujita Health University, Aichi, Toyoake, 470-1192, Japan.

Abstract

Sepiapterin reductase (SPR) catalyzes the final step of the biosynthetic pathway of tetrahydrobiopterin, which is an essential cofactor for aromatic amino acid hydroxylases and nitric oxide synthases. To aid the analysis of any possible human diseases caused by mutations in SPR, we have cloned and characterized the human SPR gene. The gene is composed of three exons spanning approximately 4 kilobases. The transcriptional starting point was determined around the cytosine nucleotide at position -81 by primer extension and RT-PCR analyses. There was no typical TATA-box within 300 bp from the transcriptional starting point. We found the Sp1-binding consensus sequence in the 5'-flanking region. The human SPR gene was mapped to chromosome band 2p13 by fluorescence in situ hybridization.

PMID:
9792819
DOI:
10.1006/bbrc.1998.9503
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center