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J Invertebr Pathol. 1998 Nov;72(3):206-13.

Expression of chromosomally inserted bacillus thuringiensis israelensis toxin genes in bacillus sphaericus

Author information

1
Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.

Abstract

Bacillus thuringiensis israelensis delta-endotoxin genes were inserted into transposon Tn917 in plasmid pTV51Ts and cloned into the chromosome of Bacillus sphaericus 2362. Many of the transformants reacted with antibody to the 135-, 128-, 65-, and 28-kDa B.t.israelensis toxin proteins and were approximately 10 times more toxic to A. aegypti larvae than the untransformed host. Some of the transformants differed physiologically and morphologically from the wild-type B. sphaericus. The toxicity of the transformed phenotype was maintained through many transfers in the absence of selective pressure.

PMID:
9784342
DOI:
10.1006/jipa.1998.4787

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