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FEBS Lett. 1998 Oct 2;436(2):189-92.

2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803.

Author information

1
Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden. birgitta@biokemi.su.se

Abstract

Aqueous polymer two-phase partitioning in combination with sucrose density centrifugation offered, for the first time, a 2D-separation method for the isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis 6803 without any cross-contaminations. The purity of the membrane fractions was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. As an initiation of a proteomics project, two prominent proteins, which were observed only in the plasma membrane (Slr1513, a hypothetical protein, and HofG, a general secretion pathway protein), or in the thylakoid membrane (PsaE, a photosystem I protein, and NdhH, a subunit of NADH dehydrogenase), were identified.

PMID:
9781676
DOI:
10.1016/s0014-5793(98)01123-5
[Indexed for MEDLINE]
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