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J Infect Dis. 1998 Nov;178(5):1416-20.

Generation and testing of mutants of Enterococcus faecalis in a mouse peritonitis model.

Author information

1
Center for the Study of Emerging and Re-emerging Pathogens, University of Texas Medical School at Houston, USA.

Abstract

A previously described mouse peritonitis model was used to study derivatives of Enterococcus faecalis strain OG1RF. The addition of sterile rat fecal extracts (SRFE) lowered the LD50 of OG1RF >10-fold. Hemolysin production caused a 35-fold lower LD50 and a much shorter survival, similar to previous results using a peritonitis model without SRFE. A purine (but not a pyrimidine) auxotroph was considerably less lethal than wild type; gelatinase mutants were also attenuated. A suicide vector was generated with an enterococcal selectable marker in order to disrupt a gene encoding an E. faecalis antigen; the resulting mutant was not attenuated despite a slower growth rate. In conclusion, this model allows attenuated mutants to be detected, corroborates prior reports that hemolysin is a virulence factor, and suggests a role for gelatinase in virulence of E. faecalis in mice; the attenuated purine auxotroph may provide a system for developing vectors for in vivo expression systems.

PMID:
9780263
DOI:
10.1086/314453
[Indexed for MEDLINE]

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