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Syst Appl Microbiol. 1998 Aug;21(3):450-3.

Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control.

Author information

1
WFM Wasserforschung Mainz GmbH, Mainz, Germany. 0613112-6430@t-online.de

Abstract

Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.

PMID:
9779610
DOI:
10.1016/S0723-2020(98)80055-6
[Indexed for MEDLINE]

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