Format

Send to

Choose Destination
Nat Genet. 1998 Oct;20(2):123-8.

A new logic for DNA engineering using recombination in Escherichia coli.

Author information

1
Gene Expression Program, European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

A straightforward way to engineer DNA in E. coli using homologous recombination is described. The homologous recombination reaction uses RecE and RecT and is transferable between E. coli strains. Several target molecules were manipulated, including high copy plasmids, a large episome and the E. coli chromosome. Sequential steps of homologous or site-specific recombination were used to demonstrate a new logic for engineering DNA, unlimited by the disposition of restriction endonuclease cleavage sites or the size of the target DNA.

PMID:
9771703
DOI:
10.1038/2417
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center