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Trends Genet. 1998 Sep;14(9):358-63.

Chimeras and mosaics in mouse mutant analysis.

Author information

1
Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada. rossant@mshri.on.ca

Abstract

As the number of mouse mutants generated by gene targeting continues to grow exponentially, the challenge is not how to generate a mutant but how to analyse the phenotype. Genes might play multiple roles in development and act in cell-autonomous and cell non-autonomous modes, making phenotypic analysis complex. Genetic mosaic analysis is a powerful tool for dissecting complex gene functions. Classical preimplantation chimeras made between mutant and wild-type embryos can answer many questions, and new genetic techniques for generating restricted genetic mosaicism promise to enhance the future power of mosaic analysis in mammals.

PMID:
9769731
DOI:
10.1016/s0168-9525(98)01552-2
[Indexed for MEDLINE]

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