Format

Send to

Choose Destination
See comment in PubMed Commons below
Am J Reprod Immunol. 1998 Sep;40(3):172-6.

Major histocompatibility complex expression on human, male germ cells: a review.

Author information

1
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.

Abstract

PROBLEM:

The male reproductive compartment is an immunologically privileged site. The expression pattern of human leukocyte antigens (HLAs) may play an important role in the maintenance of immune tolerance toward differentiating gametogenic cells. This review presents current knowledge about HLA gene expression on human, male germ cells, on mRNA and protein levels, and on their methylation status.

METHOD OF STUDY:

Different techniques were applied to study HLA gene expression in human testis: (a) protein: e.g., cytotoxicity test, fluorescent labeling techniques, enzyme-linked immunosorbent assay, and confocal microscopy; (b) mRNA: reverse transcriptase-polymerase chain reaction, Northern blot hybridization, and in situ hybridization; and (c) methylation status.

RESULTS:

In normal testicular tissue we observe a lack of HLA-class I (classical) antigens expression and inversely related expression pattern of HLA class I classical and nonclassical genes. HLA-A, -B, -C, and -E loci are likewise methylated in somatic and germ cells, whereas -F and -G genes are less methylated in sperm precursors.

CONCLUSIONS:

Immunologic tolerance in human testis is actively maintained by the specific expression pattern of HLA genes regulated by hormones and growth factors.

PMID:
9764361
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center