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FEBS Lett. 1998 Sep 18;435(2-3):237-40.

NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus.

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1
School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.

Abstract

N-Ethylmaleimide-sensitive factor (NSF) is required for multiple pathways of vesicle-mediated protein transport. Microinjection of a monoclonal anti-NSF antibody almost completely blocked brefeldin A-promoted Golgi disassembly without affecting the rapid release of beta-COP, a subunit of the Golgi coat proteins (COPI), from the Golgi apparatus. Similar results were obtained using a dominant-negative NSF which is known to compete with endogenous NSF. The present results suggest that an NSF-mediated step is present in the brefeldin A-promoted disassembly of the Golgi apparatus.

PMID:
9762917
DOI:
10.1016/s0014-5793(98)01081-3
[Indexed for MEDLINE]
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