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Inflamm Res. 1998 Aug;47(8):351-4.

Increased release of ATP from endothelial cells during acute inflammation.

Author information

1
Autonomic Neuroscience Institute, Royal Free Hospital School of Medicine, London, UK.

Abstract

OBJECTIVE AND DESIGN:

The effects of lipopolysaccharide (LPS), a potent inflammatory mediator, on the shear stress stimulated release of adenosine triphosphate (ATP) were investigated on endothelial cells from human umbilical vein in primary culture.

METHODS:

Human umbilical vein endothelial cells (HUVEC) in primary cultures were subjected to shear stress using a cone and plate apparatus. ATP released by the cells was measured by luminometry, using a luciferin-luciferase assay.

RESULTS:

Under conditions of shear stress alone (25dyn/cm2), ATP accumulates into the culture medium and reaches a maximum after 3 to 5 min of stimulation (121.7+/-13.2 pmol/ml). The shear stress-stimulated release of ATP was significantly increased after a 4 h pre-incubation of endothelial cells with 50 microg/ml (314.4+/-26.7 pmol/ml) and 10microg/ml lipopolysaccharide (207.7+/-22.2 pmol/ml). Dexamethasone, an anti-inflammatory glucocorticoid, inhibited the effects of lipopolysaccharide.

CONCLUSIONS:

These results show that non-damaged endothelial cells release ATP under experimental inflammatory conditions and support an early role of extracellular ATP in the inflammatory process.

PMID:
9754870
DOI:
10.1007/s000110050341
[Indexed for MEDLINE]

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