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Genome Res. 1998 Sep;8(9):901-15.

Comparative gene mapping: a fine-scale survey of chromosome rearrangements between ruminants and humans.

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1
Institut National de la Recherche Agronomique (INRA), Departement de Génétique Animale, Laboratoire de Génétique biochimique et de Cytogénétique, 78350 Jouy-en-Josas, France. schibler@biotec.jouy.inra.fr

Abstract

A total of 202 genes were cytogenetically mapped to goat chromosomes, multiplying by five the total number of regional gene localizations in domestic ruminants (255). This map encompasses 249 and 173 common anchor loci regularly spaced along human and murine chromosomes, respectively, which makes it possible to perform a genome-wide comparison between three mammalian orders. Twice as many rearrangements as revealed by ZOO-FISH were observed. The average size of conserved fragments could be estimated at 27 and 8 cM with humans and mice, respectively. The position of evolutionary breakpoints often correspond with human chromosome sites known to be vulnerable to rearrangement in neoplasia. Furthermore, 75 microsatellite markers, 30 of which were isolated from gene-containing bacterial artificial chromosomes (BACs), were added to the previous goat genetic map, achieving 88% genome coverage. Finally, 124 microsatellites were cytogenetically mapped, which made it possible to physically anchor and orient all the linkage groups. We believe that this comprehensive map will speed up positional cloning projects in domestic ruminants and clarify some aspects of mammalian chromosomal evolution.

PMID:
9750190
[Indexed for MEDLINE]
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