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Nat Biotechnol. 1998 Sep;16(9):857-61.

Cell penetrating PNA constructs regulate galanin receptor levels and modify pain transmission in vivo.

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  • 1Department of Neurochemistry and Neurotoxicology, Arrhenius Laboratories, Stockholm University, Sweden.

Abstract

Peptide nucleic acids (PNAs) form stable and tight complexes with complementary DNA and/or RNA and would be promising antisense reagents if their cellular delivery could be improved. We show that a 21-mer PNA, complementary to the human galanin receptor type 1 mRNA, coupled to the cellular transporter peptides, transportan or pAntennapedia(43-58), is efficiently taken up into Bowes cells where they block the expression of galanin receptors. In rat, the intrathecal administration of the peptide-PNA construct results in a decrease in galanin binding in the dorsal horn. The decrease in binding results in the inability of galanin to inhibit the C fibers stimulation-induced facilitation of the rat flexor reflex, demonstrating that peptide-PNA constructs act in vivo to suppress expression of functional galanin receptors.

PMID:
9743120
DOI:
10.1038/nbt0998-857
[PubMed - indexed for MEDLINE]
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