Differential transferrin receptor density in human colorectal cancer: A potential probe for diagnosis and therapy

Int J Oncol. 1998 Oct;13(4):871-5. doi: 10.3892/ijo.13.4.871.

Abstract

Transferrin receptor density was investigated in human colorectal surgical specimens. Crude membranes were prepared from 23 cancer tumors (adenocarcinoma or malignant villous tumor) and 3 non-cancer tumors (polyadenoma or villous tumor) and 26 adjacent control mucosa. Contrary to non-cancer tumors, Scatchard analysis of 125I-transferrin binding data evidenced higher maximal transferrin binding capacity and lower dissociation constant in cancer tissues (Bmax cancer 1.828+/-0.320 nmol/g, Kd 24.1+/-4.7 nM), as compared to paired control colonic mucosa (Bmax contol 0.851+/-0.182 nmol/g, Kd 30.7+/-7.3 nM), paired t-tests: Bmax p<0.001, Kd p<0.05). As the cancer/control Bmax ratio was 2.6+/-0.4,transferrin carrier constructs should be proposed for cancer imaging or therapy.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers
  • Colorectal Neoplasms / diagnosis
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / therapy
  • Female
  • Humans
  • Male
  • Middle Aged
  • Protein Binding
  • Radioligand Assay
  • Receptors, Transferrin / chemistry
  • Receptors, Transferrin / metabolism*
  • Transferrin / chemistry
  • Transferrin / metabolism

Substances

  • Biomarkers
  • Receptors, Transferrin
  • Transferrin