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Methods. 1998 Jul;15(3):199-205.

Double-stranded RNA-specific adenosine deaminase: nucleic acid binding properties.

Author information

1
Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, 93106, USA.

Abstract

The RNA-specific adenosine deaminase (ADAR1, herein referred to as ADAR) is an interferon-inducible RNA-editing enzyme. ADAR catalyzes the C-6 deamination of adenosine in double-stranded (ds) structures present in viral RNAs and cellular pre-mRNAs as well as synthetic dsRNA substrates. ADAR possesses three functionally distinct copies of the highly conserved double-stranded RNA binding R motif (RI, RII, RIII) implicated in the recognition of dsRNA structures within the substrate RNAs. ADAR is also a Z-DNA-binding protein. Two Z-DNA binding motifs (Zalpha and Zbeta) present in ADAR correspond to repeated regions homologous to the N-terminal region of the vaccinia virus E3L protein. Here we describe assay methods for measurement of ADAR enzymatic activity, dsRNA binding activity, and Z-DNA binding activity.

PMID:
9735305
DOI:
10.1006/meth.1998.0624
[Indexed for MEDLINE]

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