Format

Send to

Choose Destination
Immunity. 1998 Aug;9(2):169-78.

Individual variations in the murine T cell response to a specific peptide reflect variability in naive repertoires.

Author information

1
Unité de Biologie Moléculaire du Géne INSERM U277, Institut Pasteur, Paris, France.

Abstract

Previous studies have analyzed the diversity of T cell responses upon immunization. Little is known, however, about the individual variability of naive repertoires and its influence on immune responses. In the present study, T cells specific for a Kd-restricted epitope derived from HLA-A2 were purified from individual immunized mice using tetramers of MHC-peptide. Their TCRbeta chains were sequenced revealing strong biases but large variations in BJ usage and clonal composition. Most importantly, sequence analysis from nonimmunized mice demonstrated the preexistence of a small set of splenic precursors, distinct in each mouse and comprising less than 200 cells. Therefore, differences in precursor pools appear to be the major source of individual variability in antigen-selected repertoires.

PMID:
9729037
DOI:
10.1016/s1074-7613(00)80599-3
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center