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Int J Food Microbiol. 1998 Jul 21;42(3):139-45.

Isolation and partial characterization of a thermostable extracellular protease of Bacillus polymyxa B-17.

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Department of Microbiology, College of Basic Sciences, Himachal Pradesh Agricultural University, Palampur, India.


Bacillus polymyxa B-17, a sporeforming psychrotroph produced a thermostable protease. The protease was purified to homogeneity from cell free broth culture by precipitation with ammonium sulfate and gel filtration through Sephadex G-100. The enzyme had a temperature optimum at 50 degrees C and shared significant activity at 70 degrees C. The protease was also active over a wide range of pH, 5.5 to 10.0, and had optimum activity at pH 7.5. It was inhibited by metal chelating agents and has a molecular weight of 30 kDa.

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