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Int J Radiat Oncol Biol Phys. 1998 Jul 15;41(5):1171-6.

Effect of radiation on the expression of E-cadherin and alpha-catenin and invasive capacity in human lung cancer cell line in vitro.

Author information

1
Department of Radiology and Radiation Oncology, Gunma University School of Medicine, Maebashi, Japan.

Erratum in

  • Int J Radiat Oncol Biol Phys 1998 Dec 1;42(5):1181.

Abstract

PURPOSE:

To investigate the effect of radiation on E-cadherin and alpha-catenin expression in a human lung cancer cell line, and also evaluate invasive capacity in the membrane invasion culture system using the Boyden Chamber.

MATERIALS AND METHODS:

The immunoblot and immunofluorescence analyses were performed using the human lung cancer cell line A549 to examine altered expression of E-cadherin and alpha-catenin after irradiation. We also compared invasive capacity of untreated cells with that of irradiated cells.

RESULTS:

Immunoblot analysis revealed that the expression of E-cadherin increased after irradiation. In a time-course analysis, the expression was increased 6 h after irradiation with 10 Gy and reached its peak level at 24 h, being 2.3 times the control value, whereas expression at 1 and 3 h after irradiation was almost equivalent to that of the control. A slight increase in expression was observed after irradiation of 2 Gy and the expression reached peak levels after 5 Gy. After fractionated irradiation, the increase in expression of both E-cadherin and alpha-catenin was observed, and the alteration of alpha-catenin was more prominent than that after a single irradiation of the same total dose. In the immunofluorescence study for E-cadherin antibody analyzed by confocal laser scanning microscopy, increased intensity in irradiated cells produced as a nondisrupted and continuous line at cell-cell contact sites. In an invasive assay, the number of migrated cells in irradiated cells after a dose of 5 and 10 Gy was reduced significantly compared to untreated cells.

CONCLUSION:

The results indicate that irradiation of A549 increased the expression of E-cadherin, possibly preserving their functional property.

PMID:
9719129
DOI:
10.1016/s0360-3016(98)00176-x
[Indexed for MEDLINE]

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