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Oncogene. 1998 Aug 13;17(6):685-90.

Activation of NF-kappa B by oncogenic Raf in HEK 293 cells occurs through autocrine recruitment of the stress kinase cascade.

Author information

1
Institut für Medizinische Strahlenkunde und Zellforschung (MSZ), University of Würzburg, Germany.

Abstract

Raf-1 kinase has been implicated in the induction of NF-kappa B activity by serum growth factors, phorbol ester and PTK oncogenes. Here we show that Raf activation of NF-kappa B, as measured in reporter gene assays, occurs indirectly and requires the stress kinase cascade. The stress pathway presumably becomes activated through induction of an autocrine loop by activated Raf (Raf-BXB) as suramin, the tyrphostin AG1478 and a dominant negative mutant of the EGF-R blocked NF-kappa B activation. Raf-BXB synergizes with SAPKs and a dominant negative mutant of SEK significantly reduces activation of NF-kappa B consistent with a role of this signaling pathway in the activation of NF-kappa B.

PMID:
9715269
DOI:
10.1038/sj.onc.1201981
[Indexed for MEDLINE]
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