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Gene. 1998 Jul 30;215(2):471-6.

Exploiting sequence space: shuffling in vivo formed complementarity determining regions into a master framework.

Author information

1
Department of Immunotechnology, Lund University, Lund, Sweden.

Abstract

A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.

PMID:
9714846
DOI:
10.1016/s0378-1119(98)00317-5
[Indexed for MEDLINE]

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