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Cell Mol Life Sci. 1998 Jul;54(7):663-83.

Affinity capillary electrophoresis in biomolecular recognition.

Author information

1
Department of Chemistry, Ohio State University, Columbus 43210, USA. chu@chemistry.ohio-state.edu

Abstract

Affinity capillary electrophoresis is a new method for studies of biomolecular recognition. Applications reported in the literature include chiral separation of racemic biomolecules, measurement of binding constants, estimation of kinetic on- and off-rate constants, determination of binding stoichiometries (a useful tool in examining electrostatic interactions), estimation of effective charges and molecular weights of proteins, characterization of enzymatic activities and library screening for tight-binding drug candidates in solution. This technique demands only small amounts of sample (nanolitre injection volumes, picograms of proteins), involves no radiolabelled materials or chemically immobilized ligands, and does not require changes in spectroscopic characteristics upon binding. This paper reviews the most recent applications of affinity capillary electrophoresis and its use in the analysis of biomolecules.

PMID:
9711233
DOI:
10.1007/s000180050194
[Indexed for MEDLINE]

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