Format

Send to

Choose Destination
FEBS Lett. 1998 Jul 31;432(1-2):21-6.

Refolding of Escherichia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatography.

Author information

1
Max-Planck-Institut für Biophysik, Frankfurt am Main, Germany.

Abstract

Two distinctly different membrane proteins, which produced inclusion bodies in Escherichia coli, have been refolded to reconstitute properties appropriate to their native counterparts. The method employed utilises nickel chelating chromatography, where the solubilised inclusion bodies bind, refold and elute. Our aims were to release a large pool of membrane protein for functional, mutational and crystallisation screening studies. It is hoped that the methods described here will have a general application for other membrane proteins which have formed inclusion bodies.

PMID:
9710243
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center