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J Food Prot. 1998 Jun;61(6):728-30.

Gamma irradiation and ozone treatment for inactivation of Escherichia coli O157:H7 in culture media.

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Department of Food Irradiation, Korea Atomic Energy Research Institute, Taejon, Korea.


A study was conducted to investigate the reduction and elimination of Escherichia coli O157:H7 by the effects of gamma irradiation and ozone treatment. Log phase cells were found to be more sensitive to gamma irradiation than stationary phase cells. E. coli O157:H7 was found to be considerably more resistant to irradiation at -18 degrees C than at 20 degrees C. The D values for this organism for treatment with ozone in tryptic soy agar were higher than those for treatment with ozone in phosphate buffer. Gamma irradiation at a dose of 1.5 kGy or ozone treatment at a concentration of 3 to 18 ppm for 20 to 50 min was required to assure the elimination of E. coli O157:H7.

[Indexed for MEDLINE]

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