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Dev Biol. 1998 Jul 15;199(2):245-9.

GFP expression in Drosophila tissues: time requirements for formation of a fluorescent product.

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1
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.

Abstract

We analyzed the acquisition of fluorescence of a GFP fusion protein expressed from gene constructs designed to regulate either the time of translation of the protein or the time of gene transcription. Both gene constructs expressed a fusion protein with wild-type GFP appended to the N-terminus of the Bicoid (Bcd) transcription factor. One gene construct, P[gfp-bcd] expressed an mRNA that was initially translated at the time of egg deposition. GFP-Bcd fluorescence could first be detected in anterior nuclei in the embryo at syncytial blastoderm stage, within 1.5-2.5 h after translation commenced. The second gene construct, P[hs-gfp-bcd], utilized the hsp70 promoter to regulate the induction of gene expression. In larval salivary glands, GFP-Bcd fluorescence could first be detected in nuclei after a 40-min recovery period following a 10-min heat shock induction of gene expression. These estimates of the time required for the acquisition of a fluorescent GFP product in Drosophila tissues are less than suggested by previously published reports.

PMID:
9698444
DOI:
10.1006/dbio.1998.8922
[Indexed for MEDLINE]
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