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Neuron. 1998 Jul;21(1):147-54.

Differential regulation of exocytosis by calcium and CAPS in semi-intact synaptosomes.

Author information

1
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Abstract

Using a novel approach to measure exocytosis in vitro from semi-intact synaptosomes, we establish that the Ca2+-dependent release of glutamate requires cytosolic factors for mobilization from the reserve pool. The cytosolic activity for glutamate release was not satisfied by CAPS, a soluble component required for norepinephrine (NE) release. Moreover, the CAPS-independent glutamate release from synaptic vesicles (SVs) was 200-fold less sensitive to Ca2+ than that required for dense core vesicles (DCVs). The differential regulation of exocytosis by CAPS, Ca2+, and potential novel cytosolic factor(s) suggests that the docking and fusion machinery controlling DCVs has diverged from that regulating glutamate-containing SVs.

PMID:
9697859
DOI:
10.1016/s0896-6273(00)80522-x
[Indexed for MEDLINE]
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