Format

Send to

Choose Destination
See comment in PubMed Commons below
Aust N Z J Ophthalmol. 1998 May;26 Suppl 1:S77-9.

Adeno-associated virus-mediated gene transfer into human retinal pigment epithelium cells.

Author information

1
Centre for Ophthalmology and Visual Science, University of Western Australia, Perth, Australia. ylai@cyllene.uwa.edu.au

Abstract

PURPOSE:

Adeno-associated virus (AAV) is emerging as a promising vector for gene therapy

METHOD:

To determine the ability of recombinant AAV (rAAV) to express and integrate exogenous DNA into human retinal pigment epithelium (RPE) cells, a rAAV-GFP vector containing the green fluorescent protein (gfp) and neomycin resistance (neo(r)) genes was constructed and used to transduce RPE 407A cell line.

RESULTS:

Fluorescent RPE cell clones were obtained and were confirmed to still be expressing GFP after 24 passages (3.5 months).

CONCLUSION:

Adeno-associated virus-based vectors are able to efficiently transduce and stably persist in RPE cells.

PMID:
9685031
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center