Format

Send to

Choose Destination
See comment in PubMed Commons below
J Pharm Biomed Anal. 1998 Aug;17(4-5):767-73.

Determination of total cysteamine in human serum by a high-performance liquid chromatography with fluorescence detection.

Author information

1
Laboratoire Central d'Analyses, Pharmacie Centrale des Hôpitaux, Paris, France.

Abstract

A convenient, reliable and rapid method for determination of total cysteamine in human plasma by high-performance liquid chromatography with fluorescence detection is reported. This assay involves reduction of samples with dithiothreitol, derivatization of total cysteamine by addition of monobromobimane and protein precipitation by perchloric acid. The calibration curve was linear in the range 2-150 nmol ml-1 and the detection limit was 0.5 nmol ml-1. This method was successfully applied for a pharmacokinetic study of three cysteamine derivatives in healthy volunteers without any interference from coexisting substances.

PMID:
9682161
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center