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Curr Microbiol. 1998 Aug;37(2):144-8.

The effect of galE gene inactivation on lipopolysaccharide profile of Helicobacter pylori.

Author information

1
Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.

Abstract

The galE gene product, UDP-galactose 4-epimerase, mediates the incorporation of galactose in extracellular polysaccharide materials such as the O-side chain of lipopolysaccharide (LPS). The O-side chain in H. pylori LPS has been shown to cross-react with Lewis x and/or y blood group antigens, suggesting its potential involvement in H. pylori-linked autoimmune disease. To study its role in H. pylori LPS biosynthesis, the galE gene was cloned, sequenced, and a galE-knockout H. pylori strain was constructed. The H. pylori galE gene encoded a protein of 344 amino acids with a molecular weight of 39K. The LPS profile from the galE-knockout H. pylori strain showed a lower molecular weight than that of the parental strain, indicating the involvement of the galE gene in LPS biosynthesis of H. pylori.

PMID:
9662617
DOI:
10.1007/s002849900354
[Indexed for MEDLINE]

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