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J Med Virol. 1998 Aug;55(4):268-71.

Human cytomegalovirus infection induces mRNA expression and secretion of plasminogen inhibitor type-1 in endothelial cells.

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University of Western Sydney (Hawkesbury), Faculty of Science and Technology, New South Wales, Australia.


The aim of the study was to investigate whether infection of endothelial cells with human cytomegalovirus (HCMV) perturbs expression and production of plasminogen activator inhibitor type-1 (PAI-1). mRNA expression of PAI-1 was investigated by isolating total RNA from HCMV-infected and control cells, followed by Northern blotting and probing with 32P-labelled PAI-1 probe. Sandwich ELISA was used to investigate PAI-1 production. HCMV induced the expression of PAI-1-mRNA at 2-5 days postinfection (maximum expression was at 3 days postinfection which was 40% higher than control). HCMV also induced secretion of PAI-1 at 2-5 days postinfection. These results indicate that infection of endothelial cells with HCMV disturbs PAI-1 expression and production in these cells.

[Indexed for MEDLINE]

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