Format

Send to

Choose Destination
Mol Cell. 1998 Jan;1(2):309-17.

On the mechanism of RecA-mediated repair of double-strand breaks: no role for four-strand DNA pairing intermediates.

Author information

1
Department of Biochemistry, University of Wisconsin, Madison 53706, USA.

Abstract

RecA protein will bind to a gapped duplex DNA molecule and promote a DNA strand exchange with a second homologous linear duplex. A double-strand break in the second duplex is efficiently bypassed in the course of these reactions. We demonstrate that the bypass of double-strand breaks is not explained by a mechanism involving homologous interactions between two duplex DNA molecules, but instead requires the ATP-mediated generation of DNA torsional stress brought about by the action of RecA. The results suggest new pathways for the repair of double-strand breaks and underline the need for new paradigms to explain the alignment of homologous DNAs during genetic recombination.

PMID:
9659927
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center