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Am J Vet Res. 1998 Jul;59(7):842-7.

Interferon-gamma and interleukin 4 gene expression in cows infected with Mycobacterium paratuberculosis.

Author information

1
Department of Clinical Studies-New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348, USA.

Abstract

OBJECTIVE:

To determine whether clinical progression of paratuberculosis in cattle was associated with alterations in cytokine gene expression in affected tissues.

ANIMALS:

5 uninfected adult Holstein cows, 7 adult Holstein cows naturally infected with Mycobacterium paratuberculosis that did not have clinical signs of disease, and 4 adult Holstein cows naturally infected with M paratuberculosis that had progressive clinical signs of infection.

PROCEDURE:

Samples of ileum and cecal lymph nodes were obtained from each animal at the time of slaughter. A reverse transcriptase-competitive polymerase chain reaction assay was used to determine mRNA expression of interferon-gamma (IFN-gamma) and interleukin 4 in each sample.

RESULTS:

Interferon-gamma gene expression was significantly higher in ileum and cecal lymph node samples from subclinically infected cows than from clinically infected cows.

CONCLUSIONS AND CLINICAL RELEVANCE:

Progression of paratuberculosis to clinical stages is associated with reduced expression of IFN-gamma at site of infection. If immune response to M paratuberculosis can be manipulated so that IFN-gamma expression is increased, resistance to infection in cattle might be enhanced.

PMID:
9659548
[Indexed for MEDLINE]

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