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J Infect Dis. 1998 Jul;178(1):191-9.

Evaluation of an isogenic hemolysin-deficient mutant in the human model of Haemophilus ducreyi infection.

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1
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202, USA.

Abstract

Haemophilus ducreyi causes the genital ulcerative disease chancroid. One putative virulence factor of H. ducreyi is a pore-forming hemolysin that displays toxicity against human fibroblasts and keratinocytes. In order to test the role of the hemolysin in pathogenesis, an isogenic hemolysin-deficient mutant was constructed, designated 35000HP-RSM1. The lipooligosaccharide, outer membrane protein patterns, and growth attributes of 35000HP-RSM1 were identical to its parent, 35000HP. Human subjects were challenged on the upper arm with the isogenic isolates in a double-blinded, randomized, escalating dose-response study. Pustules developed at a similar rate at sites inoculated with the mutant or parent. The cellular infiltrate and bacterial load in lesions were also similar. These results indicate the hemolysin does not play a role in pustule formation. Due to the limitations of this model, the role of the hemolysin at later stages of infection could not be determined.

PMID:
9652440
DOI:
10.1086/515617
[Indexed for MEDLINE]

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